Journal: The Journal of Physiological Sciences : JPS

Article Title: Functional emergence of a column-like architecture in layer 5 of mouse somatosensory cortex in vivo

doi: 10.1007/s12576-018-0618-4

Figure Lengend Snippet: Cortical expression of C1V1, a chimeric channelrhodopsin between channelrhodopsin-1 (ChR1) and one of the Volvox-derived channelrhodopsins. a–c Excitatory neurons in culture: C1V1-Venus immunoreactivity (a), CaMKII immunoreactivity (b) and the merge (c). d–f Inhibitory neurons in culture: C1V1-Venus immunoreactivity (d), GABA immunoreactivity (e) and the merge (f). g Co-expression of CaMKII and GABA in the Venus-positive neurons (summary of 10 visual fields). Scales, 50 µm for all. ***P < 0.0005, Mann–Whitney U test

Article Snippet: Briefly, HEK293 cells were transfected with a pAAV-CaMKII-C1V1-Venus vector plasmid, pHelper and pAAV-RC (serotype DJ; purchased from Cell Biolabs Inc, San Diego, CA, USA), using a standard calcium phosphate method.

Techniques: Expressing, Derivative Assay, MANN-WHITNEY